Place it right after the moment of refusal in your edit, once the denied character is framed alone. Follow it with a dramatic dun dun dun sound for mock stakes, or precede it with a vine boom sound effect when the empty bowl reveal needs extra weight.
About this sound
A short spoken clip, just over three seconds, where a voice delivers the line 'Brother, may I have some oats?' in overly formal, pleading tones. It feels mock-tragic and absurdly desperate, turning a simple request for food into high drama. It comes from the pig meme about two brothers and a coveted supply of grain.