Put it as the final beat of a fail montage, after the last mistake is shown, so it closes the sequence rather than starting it. Lead in with No God Please No while the loss is still unfolding, then let this clip finish the moment. Follow it with Roblox Oof only if you need a comic tag after the defeat.
About this sound
This two-second clip is a breathless male shout repeating 'Game over, man. Game over,' with the second phrase landing harder than the first. It feels frantic and hopeless, like someone realising defeat is already certain. The line comes from the 1986 film Aliens, shouted by Private Hudson, played by Bill Paxton, during a moment of mounting panic.