Place it immediately after the invitation or request in the edit, then follow it with the Bruh sound and the Emotional Damage sound if the refusal needs a bigger comic payoff.
About this sound
This clip plays the French phrase Mais j'ai pas envie, meaning but I don't want to, in a slowed voice that stretches each word over about five seconds. The drawn-out delivery makes the refusal sound weary, reluctant and a little dramatic, as if the speaker is dragging out a simple no. It works as a short spoken reaction for moments of reluctance.