Place it at the peak of a scare sequence, after Phasmophobia Ghost Attack and just before the reaction shot, then follow it with Fail Sound Meme to turn the moment into a joke. FNaF 2 Hallway also works as an earlier scene-setter in the same edit.
About this sound
This clip is Mike letting out one long, drawn-out 'Aaaa' scream that lasts almost five seconds, holding the same stretched sound instead of breaking into words. It feels startling at first, then funny as the scream keeps going past the point you expect it to stop, which is why it works as a sudden shock. The overlong delivery gives it a cartoonish, over-the-top edge.