Place it in the middle of a defeat montage, after a close round and before a crowd reaction, then follow it with FNAF Jumpscare Scream for a bigger shock or No God Please No when the loss keeps getting worse.
About this sound
This is Kung Lao's scream from Mortal Kombat 3, a short 3.4-second vocal cry from the fighting game. The sharp, strained yell carries panic and pain, making it feel sudden and dramatic rather than playful. It comes from the classic arcade fighter, where Kung Lao's voice sells a heavy hit during combat.