Place this clip right after the on-screen mistake, before any commentary resumes. Lead into it with the bruh soundboard button for a flat beat, then follow with the emotional damage sound button once the character recovers and the next scene starts moving again.
About this sound
This clip is a single drawn-out vocal yell lasting about three seconds, starting on a stretched 'Nepuuuu' before sliding into a long 'ooooohhh.' The voice sounds frantic and over the top, turning a tiny outburst into full cartoon panic. It feels funny and startled at once, the kind of exaggerated reaction that makes a small mishap sound huge.