Place it at the exact reveal in an edit, then follow it with the Kiko laugh once the joke has landed. For a second interruption earlier in the same video, use Olha a Maconha Audio before the main moment so the two calls build on each other.
About this sound
This six-second clip is a loud Brazilian Portuguese shout of “Olha o Rato Borrachudo,” delivered suddenly and with exaggerated comic force. It feels absurd, startling and gleefully chaotic, turning a plain moment into a punchline before listeners can work out what the rat is supposed to be. Its silliness makes it instantly memorable.