Put it just before the reveal in an edit, then follow it with Clapping for the payoff, or use It Was At This Moment He Knew first to set up an awkward twist before this clip answers it.
About this sound
This is a short, drawn-out oooh vocal from Puss in Boots, lasting just over two seconds and delivered in a soft, catlike voice that stretches the surprise. It feels playfully surprised and gently amused, like a small gasp at something cute, awkward or unexpectedly impressive. The clip is tied to the Puss in Boots character, which is why listeners recognise it instantly as a knowing reaction.