Place it at the peak of a failed attempt, after No No Wait Wait sets up the denial, then follow with Sad Violin to close the scene and turn the disaster into a joke.
About this sound
This five-second clip is a frantic, high-pitched voice shouting 'No, NOOO! AHHHH!!!' in rising panic, stretching the denial into a full scream. It feels like comic despair, the sound of a plan collapsing in real time, funny because the reaction is far bigger than the moment deserves.