Place it right after a confident boast or taunt in a gameplay edit, then let a bigger denial take over to stretch the failure. A quick Nope works as a simple follow-up, while No God Please No gives the same refusal a second, stronger beat in the edit.
About this sound
This 1.4-second clip catches Wario mid-taunt underwater, with his sharp 'nyeh' quickly turning into a pair of panicked 'no' cries as the joke falls apart. It feels funny and frantic, like a boast collapsing in real time, and it works because the confident start and the sudden retreat sit so close together.