Place it immediately after the failed moment in your edit, then follow it with SpongeBob Fail to underline the flop, or let Vine Boom hit first for a dramatic setup before the rejection lands.
About this sound
This two-second vocal clip is a sharp, spit-like burst, like a spoken ptooey aimed at something gross or irritating. It feels instantly disgusted and playfully rude, turning a bad take or a failed moment into a clear rejection. It comes from the Thai written expression ถุย, used online as a compact dismissive reaction.