Totally Spies first aired in 2001 and followed Beverly Hills teenagers Sam, Clover and Alex, who balanced school life with missions for the World Organization of Human Protection. Sebastian's guitar theme comes from the show's exaggerated villain styling, where music helped mark a character as dramatic and self-important before he even spoke. Clips and themes from the series later circulated in nostalgia edits and soundboards, and this short riff survived because it captures the show's mix of spy action, fashion, comedy and early 2000s cartoon attitude in just a few seconds.