Place this clip right after the moment someone else claims the seat, then follow it with SpongeBob Fail for the letdown or a Vine Boom to underline the reaction shot before cutting to your reply.
About this sound
A short voice clip of someone asking, in full, 'when is it my turn to sit on it,' over about five seconds. The delivery sounds impatient and a little left out, like a kid watching everyone else get a go first. That mix of whining and hope makes it funny and awkward at the same time, especially when the wait has clearly gone on too long.