Place it at the end of a setup clip, right after the suspicious comment, then follow it with a Vine Boom for the punch and a Dun Dun Dun to underline the reveal, letting the question stay the focus of the edit.
About this sound
This is an almost nine-second voice clip of a man asking, in a slow, pointed interview tone, why are you gay. The drawn-out delivery makes the question land as awkward, teasing and deliberately confrontational, turning a simple line into a sharp reaction. It works best as a sudden callout, catching the listener off guard before the joke settles.