Place it right after the missed moment is shown, so the reaction lands on the reveal rather than before it. Open with Enemy Spotted when the target is visible, then use this clip as the payoff, and follow with Nope when the other person tries to deny what just happened.
About this sound
This three-second clip is a spoken Portuguese insult aimed at someone who has missed something obvious. The words are blunt and confrontational, with a mocking edge that makes the line feel more like a taunt than a serious argument. It lands as a sharp, funny put-down for moments of disbelief, frustration and playful trash talk.