Place this clip right after the reveal frame as the first reaction beat, then follow it with the awkward moment sound for a deflated pause, or precede it with an anime ahh to build a bigger anime-style reaction sequence.
About this sound
This clip is a single voice shouting oh my god wow in a stretched, accented delivery that drags the surprise out for about six seconds. It lands as pure theatrical disbelief, funny because the reaction is far bigger than whatever caused it, turning a small shock into an exaggerated, shareable outburst.